FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194430
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC4800.042.408631
GTCTTGC11200.036.9993631
TCGCTCT5300.036.67644
GCGTTAG850.00270530734.2120061
GCGTAGT1551.4340367E-632.832491
CCCGTCG900.003755770931.9961788
GTGTTAC5300.031.5492781
CTCGCTC5750.031.3019183
TCTGTCG4650.030.9640438
GTATAGG2859.094947E-1230.6107371
TATACTG3550.030.4201745
GTATTAA2901.0913936E-1130.082971
GTATAAT3151.8189894E-1230.0033841
GTGTTAG8050.029.8026921
GTATTAT3500.029.0802021
TTGCTCT15200.028.4188464
GTATTAG3102.7284841E-1128.1421321
TCTCGCT6650.027.065572
GTTACGC1357.95423E-426.9261131
CGCTCTG6700.026.8635865
Produced by FastQC (version 0.11.5)