FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837987
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7500.045.095868
GTCTCGC8250.036.639921
GTCTTGC17900.036.186671
TAATCCG1051.9373569E-434.2673725
CTGTCGC10550.034.1049659
TCGCTCT8850.033.3417174
GTGTAAG8750.032.901151
GTCTTAC6550.031.8651431
TCTCGCT10450.028.2375322
CGCTCTG9850.027.0312165
ATACCGT1658.860398E-526.167816
TTCACCG5500.026.1678095
GTTTCAC28100.026.1240923
GTGTTAC8000.026.0895821
GGGTTTC30900.026.0866741
TACACCG1400.001044228625.7005275
TTGCTCT24450.025.6087114
CTTGCTC23650.024.9534193
CTCGCTC11000.024.8621253
GTACGGA1751.3202884E-424.6758631
Produced by FastQC (version 0.11.5)