FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785560
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC16200.033.45271
GTCTCGC8050.033.2116781
TCTGTCG8300.031.2266588
TCGCTCT9050.029.4343344
TGTAACG1907.4205345E-626.587682
TCTCGCT8750.026.3923422
GTGTTAG11950.025.3960591
CTCGCTC8850.025.2786833
ACCGTAT1450.001277857124.825798
GGGTTTC30300.024.5628951
GTACATA4550.023.8212641
GTTTCAC27750.023.4053733
CTGTCGC10600.023.0926699
GTGTATA9750.022.9742871
GTATTGC5450.022.539141
GGGTAAG7700.022.5219211
GTTACCC6150.022.295363
GTGTAAT7800.022.2331811
GGTATAC3605.0458766E-922.0787831
GTGTAAA9850.022.0074621
Produced by FastQC (version 0.11.5)