FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725514
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7900.046.6564451
TCTGTCG7300.042.4081958
TCGCTCT9050.037.3898284
TCTCGCT9800.036.090732
CTGTCGC9850.035.0840079
CTCGCTC9600.034.586953
GTCTTGC15550.033.463441
GTGTTAG12150.033.3104361
TTCACCG5350.028.2598385
GTCTTAC5800.027.4134221
ACCGTGT6100.027.1457718
GTGTTAA5600.027.1019041
CGCTCTG10550.026.614385
GTTAGCC9800.026.5156383
GAGTGCA45750.025.3360546
GGAGTGC43500.024.4949555
GTGTTAT6200.024.479141
GTATAAC3001.7671482E-824.090581
GTGTATA8800.023.8168241
GTGTAAC4251.8189894E-1223.8071631
Produced by FastQC (version 0.11.5)