FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967854
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7850.037.6023068
GTATAAC3900.035.212111
GTCTCGC8950.033.110351
GTCTTGC19750.030.0088791
CTGTCGC11050.029.9706279
GTGTAAC5450.029.1762311
TCCGTCG1050.00797791827.4265388
GCGCAAA1606.9758855E-527.1040531
GTGTACG2756.9994712E-926.282721
GTGTTAG13400.025.3510571
GTGTAGG9800.024.3383331
GTATTGC5150.023.8585831
GAACCGT1851.92045E-423.349626
GTATAAG5300.023.1833441
GTACTAG4104.0017767E-1122.917251
GTCTTAC7600.022.8244671
GTATATA8250.022.7783571
GTTACAC7100.022.373583
GTATTAG6800.022.3209881
TTGCTCT24850.022.3087734
Produced by FastQC (version 0.11.5)