FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967854
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7950.040.744041
TCTGTCG8700.038.046428
CTGTCGC11950.030.1076839
GTCTTGC17900.029.7575661
TCGCTCT11200.029.562934
TCTCGCT10650.028.3869292
GTGTTAG13050.028.1305621
GTATTAA5100.026.8165041
CTCGCTC11050.026.7072563
CGCTCTG12100.026.1663135
GCGTAGT3109.349606E-1025.5416951
GTATTAG7050.025.5252271
GTGTAAG8100.023.9937131
GTGTAAC7500.023.9937131
GTCTCAC23200.023.5800291
GTATAGG4600.023.4721091
GTATTAT4950.023.2666321
GTATAAC3803.9835868E-1022.7308861
GTTAGCC10750.022.0959743
TATACTG4900.022.0277635
Produced by FastQC (version 0.11.5)