FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785002
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8100.040.8670738
GTCTCGC7950.040.740961
TCGCTCT8850.036.5947344
GTCTTGC15700.033.4664081
CTGTCGC11550.031.7752469
CTCGCTC10200.029.634563
GTGTAAA9700.027.4546451
GTGTTAC7350.027.4193131
TCTCGCT11450.027.0301742
GTATTAA5100.026.8144721
GTATAAG4600.026.5997141
CGCTCTG12050.025.6792375
GTGCGTA1751.3197164E-424.6773821
GTCTCAC19350.024.549851
GTATTGG4850.023.7445581
GAGTGCA44300.023.3915756
GGAGTGC40350.022.649625
GTGTTAT7650.022.580611
TGCGTAA1600.002269544422.4924052
TTCACCG4800.022.4879935
Produced by FastQC (version 0.11.5)