FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231744
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC8250.034.423641
TCTGTCG8500.033.0301638
GTCCTAC5700.029.3832041
GTCTCGC8950.029.290421
TTATACT4950.027.6556174
TCGCTCT9950.026.792524
GTCTTGC19700.026.2444971
GTATTAA5550.026.2411651
GTCCAAT7000.026.0068741
CTGTCGC11450.025.1489329
GTATAGA6750.024.8124831
TCTCGCT11450.023.9747432
ACCGTTC2151.972127E-523.4382218
GTGTAGA10100.023.0714441
GTATTAG6550.022.2348821
CTCGCTC12350.022.2275963
GTGTAAA9900.022.0664371
GTATTAC5300.021.9831681
GTCTCAC26700.021.8184991
GTCTAGC9100.021.605711
Produced by FastQC (version 0.11.5)