FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231744
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT11200.034.068284
TCTGTCG9550.033.9235658
GTCTCGC9000.033.7990841
GTCTTGC18200.031.437921
GTGTAAC5850.028.4754161
CGCTCTG11850.027.3392455
GTATACG1358.133813E-426.8246671
CTGTCGC12750.025.4094169
GTGTTAG14000.025.349311
TCTCGCT12250.025.2733972
GGGTTAC6050.025.139811
CTCGCTC13350.024.267423
GTCTCAC24950.023.8035321
GGCTAGA12600.023.5673871
GTACTAC4002.7284841E-1123.5386471
TCCGTAC2151.9711682E-523.4396953
GTGTAAA11600.023.1015871
GTATTAT7250.022.9767151
TAATACT6350.022.675074
GTATAAG5500.022.3864061
Produced by FastQC (version 0.11.5)