FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614667
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7750.038.0747641
GTCTTGC13450.035.3164251
GTACTAC2800.033.4149171
GTATAAT4750.033.333751
GTATAAC3250.033.2171971
TCGCTCT9350.033.0967254
TCTCGCT9050.031.0139922
TCTGTCG7300.030.5590678
CTCGCTC9250.029.5644653
CGTTTCG1000.00630081728.7846729
CGCTCTG9650.027.5915245
TAACACT8400.026.558934
GTGTTAG10450.025.482411
GTATTAA3851.8189894E-1124.3017581
GTGTTAC6250.024.182121
GTATATA6550.024.173331
GTCTACG1500.001559834823.9901981
GGCGTAT1851.9245986E-423.3418121
GGATACG1550.001887754223.216321
GGATAAT7150.023.151381
Produced by FastQC (version 0.11.5)