FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334886
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5400.043.9976438
GTCTCGC6200.039.607861
GGTTACG951.0522653E-438.0137371
TCGCTCT6900.035.4776884
CTCGCTC7150.034.304033
CTGTCGC7100.033.4629949
GTGTTAC4650.029.511741
GGTATAC2452.055458E-929.4800431
GTCTTGC11650.029.1384281
TCTCGCT7700.029.0431182
CGCTCTG8300.028.6249735
GTATTGG4900.028.006041
CTAGTAC2654.809408E-927.2223993
CGCACTT1400.00104114925.7129085
GTATAAA3950.025.5991231
GTCTCAC15600.025.0013431
TTGCTCT15100.024.317494
GTGTTAA4251.8189894E-1223.7921281
GTCTACA5350.022.950351
GTATATA2852.8835893E-722.8082431
Produced by FastQC (version 0.11.5)