FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334886
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG450.00992524847.977654
GTCTCGC6150.035.110861
GTCTTGC10700.033.634231
TCGCTCT6600.032.7120324
GTCTTAC5300.029.877351
TCGACAA1000.00630068828.7844335
GTGTTAC4550.026.8926011
GTATTAT2757.3123374E-926.1735461
CGCTCTG6900.026.0728575
GGTTAAC2301.1754128E-625.0355661
GTACTAG2351.4175694E-624.5028971
GTGTTAG8350.024.1360861
CTCGCTC8350.024.1324713
GTACTAA1801.5971575E-423.992421
GTATACT1801.5987817E-423.9888254
GTATTGC3352.2955646E-923.6343231
TCTGTCG6100.023.5937988
TACACTA2452.0411044E-623.4974965
GTATTAC2151.9738085E-523.4344561
GTCTCAC15350.022.9764561
Produced by FastQC (version 0.11.5)