FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880599
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8350.039.6615568
CTGTCGC10250.034.4168139
GTATTAG5550.032.5666961
TCGCTCT9100.032.437014
GTCTCGC8700.031.5784681
TAATACT3700.029.1868974
GTCTTGC17150.028.666291
TCTCGCT10000.027.4359052
GTGTTAC7450.027.1724281
GTCTTAC7400.026.3790251
GTATAAT4500.025.7059781
GTATACG1701.05150524E-425.5169641
CTCGCTC10350.025.1129593
GTCTCAC22800.024.416451
GTTTCAC28500.024.3199163
CGCTCTG11550.024.3097825
GTATTGC5100.024.0993561
GTACTAC2701.6955528E-724.0993561
GTGTAAC6000.024.0993561
ATAATAC5150.023.832933
Produced by FastQC (version 0.11.5)