FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880599
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT9200.037.552284
GTCTCGC8950.035.3891641
TCTGTCG7800.035.0573848
GTCTTGC17700.034.9756551
GGTATAC3700.029.1830141
TATACCG1000.006301842628.7839555
TACCGAT1000.006301842628.7839557
TCTCGCT11300.028.0279772
GTGTTAG12350.027.9778861
GTATTAA5150.027.9552521
CTGTCGC10500.027.4132929
GTCTTAC6850.027.3226871
TTGCTCT23300.026.874864
GTTAGCC9450.026.657483
GTATTAT4600.026.603071
GTGTTAT6550.026.37611
CTCGCTC11200.026.3480643
GTATTGG5800.026.0634541
GCGTAGG2259.699543E-725.594591
GTCTCAC22100.025.406391
Produced by FastQC (version 0.11.5)