FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250168
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10100.043.478758
GTCTTGC20900.035.6913721
GTACGGA2353.45608E-1133.8997961
GTCTCGC10400.032.729311
CTGTCGC14150.032.051829
TCGCTCT10200.028.2311384
GTGTAAC7050.026.7089331
GTATAAT7050.025.6816651
TCTCGCT12950.024.4608062
GTTCTAT8300.024.431621
TTGCTCT30100.024.395084
GTCTCAC24850.024.1893371
CTCGCTC12350.023.3164063
GTATTAC5050.022.9456791
GTGTTAG14450.022.5536561
TTACACT11350.022.1993754
GTTTCAC35500.021.9010013
CTTGCTC30100.021.7642383
CGCTCTG13300.021.6509485
GAGTAAC7100.021.420681
Produced by FastQC (version 0.11.5)