FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409402
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6400.043.8686038
GTCGTAC800.001990339536.421141
TTAAACG800.00209705536.035664
CTGTCGC8800.032.72259
GTCTTGC12350.032.4398771
GTATAGG2051.0881195E-831.9795321
GGCGTAA1402.6604463E-531.2181151
TCGCTCT7500.026.9066284
GTCTCGC6350.026.3838141
CTACGCA1400.001041738925.7105339
TTGCTCT16500.025.334164
GGTATAC2101.4999212E-524.2807581
GTATAAG3351.9990694E-923.9183561
GTCTAAC2751.8821811E-723.8392891
TAGACTA2552.8797822E-622.584945
GTACTGA3553.9090082E-922.5708451
GAGTGCA34550.022.503236
GTGTTAA3904.7475623E-1022.4130061
GTATTAT2303.0309368E-522.1693861
GTGTAGA5350.021.7846051
Produced by FastQC (version 0.11.5)