FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409402
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7400.032.105658
GTATTAA2401.5916157E-930.1926571
GTCTTGC12900.029.7713681
TACACCG1255.370354E-428.7978025
GATATAC2456.474693E-826.6188351
CTGTCGC9250.026.4619059
TCGCTCT6750.025.5980454
GTCTCGC7500.025.120291
TTGCTCT16100.025.0415674
GTGTAAA6300.023.0039311
GTCTCGA14750.022.940626
GTCTAGC4505.456968E-1222.5438521
GTACAGA5250.022.0837751
GTCTCAC16150.021.985491
GTTCGAT1650.002609224821.9582981
GTACAGC5950.021.9213941
GTGTAAG6350.021.6816581
GTATTAG3355.533002E-821.6305621
GGAGTGC35600.021.4365635
GAGTGCA38200.021.1083346
Produced by FastQC (version 0.11.5)