FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002270
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.036.8131648
GTCTCGC7950.033.691651
ATACCGT1153.2968647E-431.3000816
GTCTTGC18750.030.8870491
CTGTCGC11300.030.5799039
GGATAGG8550.027.09391
GTATTAG7150.026.324191
GTATAAA8400.024.9923111
GTACAGG10750.024.9161511
GTATATA8100.024.1305071
GTATTAT6950.023.9569051
GTGTTAG13300.023.9490741
TCGCTCT9700.023.749344
TACACCG1851.9212764E-423.348175
ATTATAC6850.023.1209373
TAATACT7200.022.9968664
TCTCGCT11650.022.8655472
TAACACT11050.022.802324
TCTGTCA27600.022.6925588
GTATAGG5200.022.2743111
Produced by FastQC (version 0.11.5)