FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177074
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9450.043.169371
TCTGTCG9750.039.8695378
TCGCTCT10900.037.669494
TCTCGCT12150.032.1083762
GTCTTGC20400.031.4247651
CTCGCTC12750.031.1640113
CGCTCTG13250.030.967855
GTCTTAC8500.029.9963631
GTATAGC4000.029.1393261
CTGTCGC13500.028.7946669
GTACTAC3950.025.8196531
GTGTTAG15650.025.6016431
GAGTGCA56800.025.3474186
GGCTACG2301.058932E-625.3385431
GGAGTGC52600.025.0447925
TACACTG13200.024.540915
GTATTAA5650.024.4976621
GTATTAG7550.024.121961
GTATAAC3702.4556357E-1023.6264781
GGTATAC5250.023.5889781
Produced by FastQC (version 0.11.5)