FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177074
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8950.033.1816251
GTATTAG6600.031.826651
GTCTTGC19200.030.5577011
TCTGTCG9350.030.0274548
GGCGTAA2354.3412E-827.7403241
TAACGGT1306.759286E-427.6880384
GTATAAG5850.027.2397841
TCGCTCT10950.026.9547464
GTCTAGT7300.025.7980771
GTACTAT4550.025.4709681
TATTAGG7450.025.1271042
GGGTAAG10100.025.1005691
GTACTAA3801.4551915E-1124.7797321
CCGTGTT7750.024.1511149
ACCGTGT9250.024.1260078
GTCTCAC29150.024.1029421
GTGTTAC9700.023.8954451
GTGTTAG14400.023.6413481
TTCACCG6150.023.4110245
GTATAGA5900.023.3259031
Produced by FastQC (version 0.11.5)