FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012571
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC16800.031.6319961
TCTGTCG8450.031.522388
GTCTTAC7200.030.332051
GTATAGA5650.029.6341441
GTGTAAC5950.029.3634621
GTCTCGC10050.028.2495521
TCGCTCT9500.027.2940944
GTATTAT5300.026.0970121
TAGTACG1450.001274680824.836584
TCTCGCT11850.024.3756162
GTGTAAA10500.024.2656421
GTATAGG5700.024.2656421
GTGTTAG14450.023.6778931
CGCTCTG11450.023.263245
CTCGCTC10250.023.2489873
TTGCTCT24200.022.9173934
CTGTCGC11550.022.4385349
GTTAGCC11350.022.2682533
GGGTTTC32750.022.2280671
GTGTAAG9750.021.6524181
Produced by FastQC (version 0.11.5)