FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012571
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8850.045.5536088
GTCTCGC8100.042.9032751
GTCTTGC17300.036.4088861
TCGCTCT10700.036.3318944
TCTCGCT10650.033.800262
CTGTCGC13900.032.111089
GGTATAC3200.031.6746831
CTCGCTC11350.030.445523
CGCTCTG11900.030.2483045
GTACGTA1204.092683E-430.1663671
GTCTCAC23100.027.8940931
GTGTTAG14100.027.727381
CTTGCTC23000.027.544373
TAGTCCG1050.00797995727.425135
GTTAGCC11550.026.8018343
TGTTAGC13750.026.703432
GAGTGCA52400.026.2409826
GTATTAT6450.025.8167951
TTGCTCT24150.025.6365364
GGAGTGC49150.025.6325935
Produced by FastQC (version 0.11.5)