FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965400
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8100.052.436518
GTCTCGC9150.040.5834851
CTGTCGC11550.038.643519
TCGCTCT10650.037.2049264
GTCTTGC18550.031.7937221
CGCTCTG11650.031.5145455
TGACGCG950.004894791630.3112057
TCTCGCT11000.030.21062
GTATAGA6050.030.0874161
GTACCGA1000.005949883329.124621
CTCGCTC10950.028.3692953
GGTATAC3750.027.182981
GTTAGCC11000.026.9268383
GCGCTAC1906.9254074E-626.8253081
AGTCGTA1358.4264367E-426.6626347
GTGTTAG13050.025.6653731
GTATAAC3801.2732926E-1124.9092141
GTACTAG2951.366061E-824.681881
GTATAGC4151.8189894E-1224.5629331
ACCGTGT6550.024.179558
Produced by FastQC (version 0.11.5)