FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965400
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC16400.036.6424561
TCTGTCG8900.033.9733778
CGTAAGG1708.3236955E-833.8808022
GTCTCGC8500.030.6643641
TCGCTCT8950.029.7624874
GTGTTAG11500.027.0720391
CTGTCGC11800.026.8441839
TAGTACT4100.026.3389174
GTACTAT4950.026.3279881
TAACACT9150.025.1778954
TTGCTCT23400.024.6130074
GGATAAT8150.023.9859281
GTGTAAG10100.023.656091
CTCGCTC10700.023.5491283
GTCCTAT5900.023.3158871
GGAGTGC42250.023.1735025
GTGTTAT7500.023.168631
GTATTAG6250.023.168631
CTTGCTC23150.023.0128943
GTATAAG5050.022.939241
Produced by FastQC (version 0.11.5)