FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4220148
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17150.043.2356958
GTCTCGC17350.034.5461431
CTGTCGC22250.033.9725889
GTCTTGC40150.033.3158381
TCGCTCT18700.030.0277184
TCTCGCT22150.026.9949532
CTCGCTC20550.025.9416793
GTTTCAC66450.025.8024043
GGGTTTC72150.025.023371
CGCTCTG21000.024.3393175
GTATAAC8450.024.2206921
TTGCTCT57200.024.0384644
GTGTTAC19300.023.8598921
TTCACCG13450.023.550485
GTGTAAA28350.023.462461
ACCGTGT14150.023.4029588
GTATTAT15050.023.3125571
GTGTAAC14750.022.7955991
CTTGCTC53600.022.7143383
GTATATA19050.022.6382161
Produced by FastQC (version 0.11.5)