FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4220148
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17750.042.5818528
GTCTCGC18000.042.427511
TCGCTCT20250.034.8369264
GTCTTGC39750.032.6981241
CTGTCGC23250.032.5087249
CTCGCTC21950.029.8432163
CGCTCTG22700.029.1736285
TCTCGCT23650.028.9738922
GTATCGC1804.259424E-628.5569761
GTATTAG15650.027.2144991
GTATTAC10950.025.483331
TTGCTCT56400.025.1435094
GTACTAG8300.024.7723161
TACCCCG3602.0190782E-1023.9942515
CTTGCTC52850.023.0186823
GTATAGG12450.022.4130481
GTGTAAA27250.022.3665121
GTTTCAC63700.022.1490633
GGGTTTC69400.022.1143151
GTCTCAC51400.022.0010951
Produced by FastQC (version 0.11.5)