FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428830
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10750.040.851478
GTCTCGC11300.036.5267751
TCGCTCT12450.034.6951074
GTCTTGC21850.033.140841
GTATTAC5400.030.8424631
GTATAGG6250.030.1236951
TCTCGCT14350.028.0963122
CTGTCGC16600.027.7560889
CGCTCTG13600.026.4677755
GTACTAT5150.025.3093051
GTGTTAC10450.024.9460121
CTCGCTC14450.024.9108493
CGAACGG1450.001278242224.8249475
GGTATAC5600.024.5686071
GTTTCAC34000.024.3503533
GTCTCAC27150.024.2709351
TTGCTCT30800.024.0753654
GTATTAG7850.023.9838331
GGGTTTC39400.023.341161
GTGTTAA9800.022.9060691
Produced by FastQC (version 0.11.5)