FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417833
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18200.042.3229528
GTCTCGC18250.035.21691
CTGTCGC22300.034.864429
GTCTTGC42800.033.4459571
TCGCTCT20550.030.1266044
TCTCGCT22900.028.3142432
GTATTAT15700.027.4462931
GTATTAG16350.026.3551581
GTATAAT15800.026.3480871
CTCGCTC23300.026.2821543
GTCTCAC55050.025.0747031
GTATTAC12300.024.9387991
TAATACT14500.024.3271734
TTGCTCT59600.024.157244
GTCTTAC17750.023.453511
GGGTTTC78800.022.8929651
GTTTCAC70800.022.7935893
GTACTAC8050.022.6816921
GTATAAC9150.022.3495241
CGCTCTG24200.022.3105095
Produced by FastQC (version 0.11.5)