FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417833
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17950.039.3016938
GTCTTGC42750.034.4998321
GTCTCGC20200.033.7822531
CTGTCGC22900.033.3211569
TCGCTCT20750.030.5291164
CGCTCTG23500.026.3433135
TCTCGCT24950.026.3117962
CTCGCTC23800.026.0118453
GGTATAC8700.025.3022461
GTTTCAC68450.024.2933943
GTGTAAC15000.023.969661
GGGTTTC75000.023.8718221
TTGCTCT58350.023.5636274
GTGTAAG23300.023.3041291
GTGTTAG29900.023.0682011
CTCGATC17850.022.987218
GTACTAT11550.022.8706021
GTATTAT16200.022.647071
CTTGCTC55350.022.4997733
GTGTTAC20250.022.1035421
Produced by FastQC (version 0.11.5)