FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4172695
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15800.038.2727058
GTCTCGC18100.035.9331861
TCGCTCT20950.032.6442384
CTGTCGC19750.030.2536669
CGCTCTG22700.029.4933475
TCTCGCT23200.027.949932
GTCTTGC38800.027.6866151
CTCGCTC23800.026.9425833
GTGTAAA27550.025.4644281
GGTATAC9550.024.4867361
GTATTAA13950.024.0972731
GTCTCAC50950.023.9528141
GTATAAT13000.023.0475521
GTATTAG16000.022.3800161
TTACACT20200.022.0956764
GGATAAG18650.021.9428731
GGCTAGA24850.021.4674641
TCTGTCA56000.021.2110868
GTCTTAG18850.020.5470161
TTGCTCT52850.020.4321124
Produced by FastQC (version 0.11.5)