FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027919
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17450.042.0762868
GTCTCGC17750.037.2217641
CTGTCGC20750.036.078459
TCGCTCT19450.034.7889144
GTCTTGC38150.030.21051
CGCTCTG22150.027.948085
CTCGCTC22350.027.6983283
TCTCGCT23750.026.4218082
GTAACGC2051.36510425E-524.5797143
ACCGTGT12450.023.1272138
GTACTAC7750.022.7332941
GTGTTAG28700.022.5088631
GTATTAG14050.022.4669931
GTATTAC8700.021.93851
GTGTAAA25500.021.8789371
TTGCTCT55000.021.856794
GGGTTTC68800.021.6600971
TTCACCG12650.021.6232195
GGTATAC8650.021.2166441
GTATAAC7700.020.9741691
Produced by FastQC (version 0.11.5)