FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986648
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13250.043.8900031
TCTGTCG11900.038.7171368
TCGCTCT15700.034.3888244
TCTCGCT17550.031.1739712
GTCTTGC26550.029.9442621
CTCGCTC17750.028.3893433
CGCTCTG17100.027.784565
GTGTTAC11400.024.5376591
GTCTCAC31400.024.3813691
GTACTAG5900.023.7058731
CTGTCGC19800.023.6349549
GTACTAT5700.021.9547481
GTCTTAC11450.021.8588751
GTACGAA1700.002832462321.6508751
TTGCTCT35500.021.494794
GGGTTTC47950.021.3393211
GCGCGAT8000.020.6987659
TTCACCG9400.020.6771935
GTTTCAC46550.020.4131473
GTATTAG9800.020.2811261
Produced by FastQC (version 0.11.5)