FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4251595
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16100.042.0311138
GTCTCGC17800.037.3246421
CTGTCGC20050.033.0325749
TCGCTCT20900.029.9669044
GTCTTAC17050.029.5460151
GTCTTGC41000.028.6692451
CTCGCTC23500.026.9748863
TCTCGCT23850.026.8810632
GTTTCAC65250.026.7165073
GGGTTTC76000.025.8412131
TTCACCG12550.025.8129545
CGCTCTG22650.025.7445765
ACCGTGT15250.025.0192898
GTGTTAC19900.024.5807951
GTGTAAA26100.024.336211
GATCGTT5050.022.8085449
TTGCTCT57750.022.4382744
GGTTTCA72500.022.2563952
GTATAGA12500.022.1946241
GTATTAG15200.022.0947191
Produced by FastQC (version 0.11.5)