FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008798
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16300.036.2157448
GTCTCGC17200.032.7052761
GTCTTGC37250.032.360351
CTGTCGC20000.030.9556279
GTATTAG15200.030.7603991
TCGCTCT19400.030.0576114
GTATAAC8950.028.5693651
GCGCTAA2207.011877E-726.56581
GTATTAA12250.026.2405031
CTCGCTC20850.024.8750023
TCTCGCT21500.023.7879262
CGCTCTG22400.023.4609725
TATACCG1851.921807E-423.3480555
GTGTTAG30900.023.1698471
GTCTTAC21950.022.9651951
TTACACT22100.022.8022084
GGGTTTC68100.021.9918771
GTATAAG13450.021.7266751
GTCTCAC46900.021.4962061
GTGTAAG21450.021.4569911
Produced by FastQC (version 0.11.5)