FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008798
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18450.038.6269728
GTCTCGC18000.035.893681
CTGTCGC23350.032.3708659
TCGCTCT19850.029.3741664
GTCTTGC37000.029.3675581
GTGTTAC16650.028.2210751
CGCTCTG20100.027.9340675
CTCGCTC23300.025.0247733
GTATAGA13350.024.7479421
GGTATAC8350.024.6195091
TCTCGCT24500.024.4336342
GTATTAG14850.024.2257651
GTGTAAC13350.023.098081
TTACACT21700.022.2257334
GTATTAC8650.022.0681081
GTATAAT13350.021.998171
GTACTAC7700.021.9303191
GTATAAC8450.021.7215671
TAACCCG4659.094947E-1221.6725865
GGTTAGA15250.021.6645931
Produced by FastQC (version 0.11.5)