FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158404
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17500.039.07958
GTCTCGC18250.034.431771
GTCTTGC37150.033.239281
CTGTCGC23100.031.1638749
TCGCTCT20300.030.4976124
TCTCGCT22750.028.4971492
GTATAGA12600.027.2553061
CTCGCTC21450.026.5301743
CGCTCTG21150.025.5278575
GTTTCAC64350.024.6271653
GGGTTTC70600.024.3213061
GTGTTAC16600.023.7689171
TACACTG25250.023.6635655
TTGCTCT56350.022.3566934
TTCACCG12850.021.8486655
GTCTTAC17400.021.8362391
GTATTAT14150.021.6878551
GTATTAC10450.021.67551
GTGTAAG20000.021.5548881
GTGTAAT17100.021.3647421
Produced by FastQC (version 0.11.5)