FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743954
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10100.038.3600581
TCTGTCG10550.035.481418
TCGCTCT11900.033.8759164
GTCTTGC26600.033.8024561
GTATTAG9300.028.2972551
TCTCGCT13950.027.3526332
GGTATAC6250.026.9012581
CTCGCTC13550.026.0319563
CGCTCTG14050.025.617915
GTATAAC4850.024.1158731
GTATTAC7000.024.0189781
TTGCTCT39000.023.995444
GTTAGCC15050.023.4374083
GTTTCAC40300.022.8641413
GTACTAC5450.022.8022231
GGGTTTC46200.022.6265771
GTGTTAG18750.022.612651
GTATTAA9100.022.4926911
CTTGCTC35600.022.2429643
TAATACT7850.022.0085584
Produced by FastQC (version 0.11.5)