FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743954
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.033.9832088
GTATTAG7900.032.625891
GTATAGG7100.031.116041
GTATTCG1901.8127866E-731.0068631
GTCTCGC11550.029.9665891
GTCTTGC26550.028.8463081
GTCTTAC10550.025.8268051
TCGCTCT12400.024.3825384
GTATAGT6700.024.1807231
GTGTAAC9150.024.1446881
ACCGTGT9600.023.9942048
GTTTCAC41100.023.4700663
TTCACCG8000.023.3956265
GGGTTTC45900.023.263591
GGTATAC6500.022.6588611
GAGTGCA66450.022.640546
CCGTGTT8950.022.521759
GTGTAAA18150.022.3155441
GTGTTAT10900.022.2950731
GTATTAC6650.022.1477581
Produced by FastQC (version 0.11.5)