FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418873
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8700.034.7528468
GTCTACG2558.185452E-1131.508951
GTCTCGC10900.030.8256841
GTCTTGC22350.028.106211
GTGTAAT10700.027.3059711
GTCGTAG1357.74084E-427.0531371
TCGCTCT11750.026.9572244
TTCACCG9100.026.8966335
TCTACGG2207.9670644E-726.1790942
GGGTTTC44750.026.11611
GTATTAA8700.025.1874031
ACCGTGT8950.024.9344068
GTATTAG9100.024.8829421
GTATAAT7650.024.8252321
GTTTCAC38650.024.0270583
GTGTAAC8000.023.7391281
CCGTCCG2502.42635E-623.0361739
GCGTAGT2552.5395075E-622.9155981
TCTCGCT13850.022.8712642
GGCGTAC1600.002083592622.8260841
Produced by FastQC (version 0.11.5)