FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869036
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.039.373168
GTCTCGC9850.038.826881
GTCTTGC27650.034.5790941
TCGCTCT12450.032.3813824
GTATAAC6100.030.1422421
TATACCG1204.231074E-429.9961475
CTCGCTC14000.026.739893
CGCTCTG14400.026.4965955
TCTCGCT14600.026.1340852
GTATTAC6850.025.7683181
TAATACT8950.025.7397124
GTGTTAG20750.025.1655041
GTATTAT10350.024.160391
CTGTCGC18700.023.4852529
GGTATAC6350.023.1644331
GTATAAG9450.022.5700021
GTCTTAC11750.022.5335731
TTGCTCT37050.021.3737724
TATACTG9150.021.2431745
GTGTAGC13600.021.0907041
Produced by FastQC (version 0.11.5)