FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043997
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7950.045.939371
TCTGTCG8800.043.360378
TCGCTCT11050.034.5313384
TCTCGCT11500.033.805322
GTCTTGC19900.033.7688981
CTCGCTC11700.031.996813
GTATACG1153.0276383E-431.7580871
TACGGTC1153.2957102E-431.301997
GTATTAG6800.030.0767771
GTATAAG5300.028.9418051
CGCTCTG12400.027.8688725
CTGTCGC13800.027.6521229
GTACCGA1100.009327956526.561311
GTACGGA2301.0344502E-625.406471
ACCGTGT6250.025.3420938
GCGCTAC1450.001174593125.1874491
TACGCCT3201.3478711E-924.7481384
GGTATAC4201.8189894E-1224.3478661
GTGTTAC8800.024.0711881
GTCTCAC22500.024.023231
Produced by FastQC (version 0.11.5)