FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870265
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5400.028.5182118
CGGTCTA500.007211882627.999728
TGTCGCC6700.027.16461410
GCGAATG2150.026.04693270
GTCTCGC5950.025.8841511
CTGTCGC7000.025.00049
GTATTAG3300.023.3349551
TCTCGCT6500.023.1548372
GTCGCCC6850.022.99306911
TCGCTCT6550.022.9780834
TAACGCT800.00266710421.8759354
CTCGCTC6950.021.1519833
GTGTAAC3200.020.7826941
TCGCCCA7750.020.32290512
GTATATA3650.020.1383881
CGAATCT4350.020.11526570
GTGTTAC4650.019.571251
GGCGAAT9500.019.52662870
GTCTTGC12250.019.4299221
GTCTCAC14650.019.352881
Produced by FastQC (version 0.11.5)