FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870265
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6050.027.1903328
TGTCGCC6600.024.92380510
GTACGGA1151.4975012E-624.351
GTCTCGC6050.024.2996921
TCGCTCT6500.024.2322864
GTATTAG3700.023.6507641
CTGTCGC6850.022.9922969
GTCGCCC6550.022.97669411
TCGCCCA7400.021.2834112
CTCGCTC7450.020.2031483
GTGTAGG5900.019.5780221
TCTCGCT7700.019.0926152
GTATTAT3001.8189894E-1218.6683351
GTCTTGC12200.018.6492081
CGCTCTG8850.018.5887365
GTATAAT3400.018.5310671
GTGTTAC4800.018.2307971
GTACTAG2901.8189894E-1118.1050661
GGGTAAC3700.017.974581
GTCTTAC4500.017.8904881
Produced by FastQC (version 0.11.5)