FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904873
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.002419644335.0005535
GCGTAAC500.007205444428.004851
TCTGTCG4600.025.8692918
GTATTAT2700.024.6338981
CTGTCGC5700.023.333099
GTCGCCC5000.023.09975611
ATCTCGT3950.023.03773170
TGTCGCC5850.021.53823710
CACGAGC5750.021.30412570
GTATATA5150.021.071611
GTATAGC2001.0677468E-921.0036371
GTATTAG3500.020.0034661
GTCTCGC6000.019.836771
GTGTATA4950.019.801411
TCGCTCT6250.019.04034
GTTATAC2104.2757165E-818.333623
GTATAGA3650.018.2223361
GTATTAC2155.586662E-817.9100781
GTCTTGC11100.017.6607171
GTCTAGA5750.017.6552331
Produced by FastQC (version 0.11.5)