FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159163
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG604.9834774E-429.1689155
TCTGTCG5500.025.454158
TGTCGCC6200.023.14587410
CGTTTCG800.002668139321.8746627
CTGTCGC7250.020.7592589
GTCTTGC12350.020.6903341
TCGCTCT6600.020.6834134
GTATAAG3900.020.6430951
GTATTAG3700.019.8668561
GTCGCCC7200.018.4727911
GTATAGA3350.017.7629741
CGTATCC1401.8655347E-417.5013483
CTCGCTC7450.017.383893
TCGCCCA7350.017.14338312
CGCTCTG7150.017.1341865
GTCTCGC6450.016.8233911
GTGTAGG5850.016.7538171
GTAATAC2301.2080272E-716.7404213
TCTCGCT7400.016.5553282
TTATACT3005.5479177E-1016.3345934
Produced by FastQC (version 0.11.5)