FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701481
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3250.029.0767298
CTGTCGC4650.025.5912259
TCGCTCT4150.025.3010354
TTTAGCG700.001226845224.9998323
GTACTAA2550.023.3345471
TGTCGCC4650.023.33317610
GTATTAG3400.022.648241
GTCTCGC4850.021.6506121
GTCTTGC9800.021.4296881
CGCTCTG4850.020.9276945
GTCGCCC4700.020.85092411
TCGCCCA5300.020.47156112
CTCGCTC5050.020.0988753
GCCGTAT900.00527809219.44431363
GTTCATA3650.019.179081
GTCTAAG4100.018.7814661
TCTCGCT5450.018.6237282
GCACCGA1702.1211326E-618.5292876
GTGTTAG6850.018.3951171
GTATTCG1000.00968932217.5009121
Produced by FastQC (version 0.11.5)