FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823083
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4800.025.5201748
CTGTCGC5250.023.3333689
TGTCGCC5550.021.44147510
GTCGCCC4950.021.21215411
GTATAGA3150.018.8899561
TTATACT3150.018.888924
TCGCCCA5450.018.62388212
GTATAAT3600.018.4732671
GTCTTGC10850.018.3881361
TAGCGCT1150.001008316218.2608974
GGCTAGA7250.017.8630771
GTATTGA3600.017.5009881
CATACGG1000.00969240217.5000275
GTCTCAC13050.016.8975051
TCGCTCT4150.016.8674964
GTATTAA4550.016.1547581
GTGTAGG6950.016.1160161
GTATTAG4100.015.366721
GTATAAG3653.45608E-1115.3433311
GTCTTAC3905.456968E-1215.2572721
Produced by FastQC (version 0.11.5)