FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823083
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT808.673548E-526.2499396
TCTGTCG3700.024.5945368
TCGCTCT5350.024.2055514
GTCTCGC4700.023.0869521
GTATTAG3350.022.9869121
CTGTCGC4600.022.0651669
GTATTAA3750.020.5349751
CGCTCTG5700.019.6490765
CGCCGTA1257.831365E-519.59941768
GTATTAC3150.018.89041
CTCGCTC5950.018.8245183
TGTCGCC4900.018.57138410
GTGTATA5700.018.4225251
GTCGTAT950.0072088618.4225231
GTACTAT2306.0790626E-918.262331
TCGCCCA5450.017.9816112
TTACACT7100.017.7464374
GTATAAT4000.017.50141
TCCCGTA1000.00969261517.4999612
GTGCGAC1000.00969261517.4999611
Produced by FastQC (version 0.11.5)