FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152629
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6000.025.6666681
TACACCG750.001833547723.3333345
TCGCTCT6550.022.4427494
ACCGATC1102.9249903E-522.2727268
TCTGTCG6150.021.6260178
GTGTAGG6050.020.8264451
TCTCGCT7250.020.7586212
AACGGAT850.003791824420.58823632
GTATTCG1055.4686895E-420.01
CACCGAT1751.2667806E-720.07
TGTCGCC7050.019.85815410
TAATAGT3700.018.9189194
GTCTTGC13150.018.8973391
CTGTCGC7600.018.4210519
GCCGTAG1150.00100840718.26086812
TATAATA3700.017.9729732
TCGCCCA7600.017.96052612
GTGTTAG8600.017.9069771
GTCGCCC7250.017.8620711
CGCTCTG7650.017.8431385
Produced by FastQC (version 0.11.5)