FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908272
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2800.027.5040451
TCTGTCG4800.023.3330978
GTCTCGC4950.021.9224171
TCGCTCT5300.021.7928034
GTACGAT900.005273628519.4473061
CTGTCGC6700.019.3281639
TGTCGCC6350.019.29114310
GTACTAG2203.4997356E-919.0937161
ATAATAC3700.018.9192243
GTCTTGC12100.018.515121
TATAGGG2659.458745E-1118.4913482
TCTCGCT6150.018.2121522
GTACAGA6100.017.7895011
GTCGCCC6100.017.78670511
CTCGCTC6500.017.7695163
GTATTGG3400.017.5025751
GTGTAGA5850.017.352981
TGGACCG1653.397119E-516.969975
GGATAGG5600.016.8774811
TCTCCGC6050.016.77669170
Produced by FastQC (version 0.11.5)